Product Info Summary
SKU: | A00015-4 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Apolipoprotein E ApoE Antibody
SKU/Catalog Number
A00015-4
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Apolipoprotein E ApoE Antibody catalog # A00015-4. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Apolipoprotein E ApoE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00015-4)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
PI9-17
Isotype
IgG
Immunogen
Synthesized peptide derived from human ApoE.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with dog p53.
Reactive Species
A00015-4 is reactive to APOE in Human, Mouse, Rat
Applications
A00015-4 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36.154kDa
Background of APOE
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-Apolipoprotein E ApoE Antibody A00015-4
Western Blot (WB) analysis of 293T cells using ApoE polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For APOE (Source: Uniprot.org, NCBI)
Gene Name
APOE
Full Name
Apolipoprotein E
Weight
36.154kDa
Superfamily
apolipoprotein A1/A4/E family
Alternative Names
APOE; Apo-E; Apolipoprotein E; apolipoprotein E3; late onset); MGC1571 APOE AD2, APO-E, ApoE4, LDLCQ5, LPG apolipoprotein E apolipoprotein E|apolipoprotein E3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APOE, check out the APOE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APOE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Apolipoprotein E ApoE Antibody (A00015-4)
Hello CJ!
A00015-4 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xue Y,Huang S,Huang J,Li S,Zhang C,Zhou X.Identification of Apolipoprotein E as a Potential Diagnostic Biomarker of Nasopharyngeal Carcinoma.Cancer Manag Res.2020 Sep 24;12:8943-8950.doi:10.2147/CMAR. S239479.PMID:33061590;PMCID:PMC7522425.
Species: Human
A00015-4 usage in article: APP:WB, SAMPLE:NPC TISSUES, DILUTION:NA
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